[血清中地高辛放射免疫化学测定的误差来源]。

Nuclear-Medizin Pub Date : 1975-12-15
H Müller, E H Graul
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引用次数: 0

摘要

在一项有大量血清样本的研究中,我们比较了5种地高辛RIA,并通过同时测定评估了试验的可靠性。对同一种血清,不同的RIA测定结果有相当大的差异;在回收率和重现性方面也记录了明显的差异。在其他一些误差来源中,观察到温度和葡聚糖-木炭分离持续时间的依赖性;因此,建议在4℃下进行木炭培养。每个能够测定血清中地高辛浓度的实验室都应与临床医生合作,声明其所使用RIA的治疗和毒性水平范围。
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[Sources of error in the radioimmunochemical determination of digoxin in serum].

In a study with numerous samples of serum we compared 5 Digoxin RIA and evaluated the reliability of tests by simultaneous determinations. There were found considerable differences in the determinations with the various RIA for the same serum; evident differences also were recorded in the recovery and in the reproducibility of the assays. Among some other sources of error the dependence of temperature and of the duration of the dextran-charcoal separation were observed; therefore, the charcoal incubation is recommendable at 4 degrees C. Each laboratory which is able to perform the determinations of digoxin concentrations in the serum should declare its own range for the therapeutic and toxic levels for the used RIA in collaboration with the clinicians.

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