{"title":"cas15通过调节MiR-139-5p/caspase-3促进年龄相关性白内障晶状体上皮细胞凋亡","authors":"Tengfei Wu, Y. Liu, Xiuquan Guo","doi":"10.31491/csrc.2019.06.032","DOIUrl":null,"url":null,"abstract":"Objective: The aim of this study was to reveal the role of CASC15 and miR-139-5p function in regulating agerelated cataracts (ARC). Methods: The expression of CASC15, miR-139-5p and caspase-3 were detected by qRT-PCR. Western blot was used to analyze the level of apoptosis-related proteins. Flow cytometry was used to measure the apoptosis of SAR01/04l cells treated with UV irradiation. Results: The expression of CASC15 and caspase-3 in the lens of ARC was higher than that in normal lens, but the expression of miR-139-5p had opposite trend. The level of caspase-3 and Bax were increased and Bcl-2 was decreased in ARC group. In addition, CASC15 negatively regulated miR-139-5p expression and promoted SAR01/04l cell apoptosis. CASC15 could combine with miR-139-5p, and miR-139-5p could combine with caspase-3 3’-UTR. Furthermore, pcDNA-caspase-3 could reverse the inhibitory effect of si-CASC15 and miR139-5p mimic on SRA01/04 cell apoptosis. Conclusion: CASC15 promoted lens epithelial cell apoptosis by regulating miR-139-5p/caspase-3. Keywords: ARC; CASC15; LECs apoptosis; miR-139-5p","PeriodicalId":158678,"journal":{"name":"Clinical Surgery Research Communications","volume":"14 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"CASC15 Promotes Lens Epithelial Cell Apoptosis in Age-Related Cataracts by Regulating MiR-139-5p/caspase-3\",\"authors\":\"Tengfei Wu, Y. Liu, Xiuquan Guo\",\"doi\":\"10.31491/csrc.2019.06.032\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: The aim of this study was to reveal the role of CASC15 and miR-139-5p function in regulating agerelated cataracts (ARC). Methods: The expression of CASC15, miR-139-5p and caspase-3 were detected by qRT-PCR. Western blot was used to analyze the level of apoptosis-related proteins. Flow cytometry was used to measure the apoptosis of SAR01/04l cells treated with UV irradiation. Results: The expression of CASC15 and caspase-3 in the lens of ARC was higher than that in normal lens, but the expression of miR-139-5p had opposite trend. The level of caspase-3 and Bax were increased and Bcl-2 was decreased in ARC group. In addition, CASC15 negatively regulated miR-139-5p expression and promoted SAR01/04l cell apoptosis. CASC15 could combine with miR-139-5p, and miR-139-5p could combine with caspase-3 3’-UTR. Furthermore, pcDNA-caspase-3 could reverse the inhibitory effect of si-CASC15 and miR139-5p mimic on SRA01/04 cell apoptosis. Conclusion: CASC15 promoted lens epithelial cell apoptosis by regulating miR-139-5p/caspase-3. Keywords: ARC; CASC15; LECs apoptosis; miR-139-5p\",\"PeriodicalId\":158678,\"journal\":{\"name\":\"Clinical Surgery Research Communications\",\"volume\":\"14 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-06-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical Surgery Research Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31491/csrc.2019.06.032\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Surgery Research Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31491/csrc.2019.06.032","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
目的:本研究的目的是揭示cas15和miR-139-5p功能在调节年龄相关性白内障(ARC)中的作用。方法:采用qRT-PCR检测cas15、miR-139-5p、caspase-3的表达。Western blot检测细胞凋亡相关蛋白水平。流式细胞术检测紫外线照射后SAR01/04l细胞的凋亡情况。结果:ARC晶状体中cas15和caspase-3的表达高于正常晶状体，而miR-139-5p的表达则相反。ARC组caspase-3、Bax水平升高，Bcl-2水平降低。此外，CASC15负调控miR-139-5p表达，促进SAR01/04l细胞凋亡。cas15可与miR-139-5p结合，miR-139-5p可与caspase-3 3′-UTR结合。此外，pcDNA-caspase-3可以逆转si-CASC15和miR139-5p mimic对SRA01/04细胞凋亡的抑制作用。结论:cas15通过调控miR-139-5p/caspase-3促进晶状体上皮细胞凋亡。关键词:弧;CASC15;lec细胞凋亡;mir - 139 - 5 - p
CASC15 Promotes Lens Epithelial Cell Apoptosis in Age-Related Cataracts by Regulating MiR-139-5p/caspase-3
Objective: The aim of this study was to reveal the role of CASC15 and miR-139-5p function in regulating agerelated cataracts (ARC). Methods: The expression of CASC15, miR-139-5p and caspase-3 were detected by qRT-PCR. Western blot was used to analyze the level of apoptosis-related proteins. Flow cytometry was used to measure the apoptosis of SAR01/04l cells treated with UV irradiation. Results: The expression of CASC15 and caspase-3 in the lens of ARC was higher than that in normal lens, but the expression of miR-139-5p had opposite trend. The level of caspase-3 and Bax were increased and Bcl-2 was decreased in ARC group. In addition, CASC15 negatively regulated miR-139-5p expression and promoted SAR01/04l cell apoptosis. CASC15 could combine with miR-139-5p, and miR-139-5p could combine with caspase-3 3’-UTR. Furthermore, pcDNA-caspase-3 could reverse the inhibitory effect of si-CASC15 and miR139-5p mimic on SRA01/04 cell apoptosis. Conclusion: CASC15 promoted lens epithelial cell apoptosis by regulating miR-139-5p/caspase-3. Keywords: ARC; CASC15; LECs apoptosis; miR-139-5p
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
