Assessment of Antibacterial Activity in Vitro Eco-Friendly Synthesis and Characterization of Silver Nanoparticles

Silver nanoparticles (AgNPs) are one of the most essential and interesting nano materials between different metallic nanoparticles that are implicated in biomedical utilization. The expansion number of resistant bacteria create an inquiry for new antibiotic methods. Metallic nanoparticles have derived as a new platform against different microorganisms. The nanoparticles can by oxidative stress damaging the membrane of bacteria and DNA. Synthesis novel sliver nanoparticles using different reducing and stabilizing agents and Study antibacterial activity of the synthesized silver nanoparticles. Silver nanoparticles (AgNPs) were synthesis by utilizing green, simple and easy approach chemical reduction method using glucose as reducing and gelatin as stabilizing agent. The optimum conditions of AgNPs synthesizing were obtained by varying the concentration of AgNO3, concentration ratio of glucose /AgNO3 and temperature. The synthesized AgNPs were determined by UV—vis spectrum. Atomic Force Microscopy (AFM), Scanning electron microscopy (SEM), and Fourier transmission infrared spectroscopy (FTIR) analysis. Chemistry method for producing. The obtained AgNPs with particle size 75.7nm. Silver nanoparticles (AgNPs) showed excellent antibacterial against Gram -negative bacterial strains Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Gram-positive bacteria, Staphylococcus aureus, Bacillus cereus.