Impact of Modern Analytical Tools In The Standardization of Kharaliya Rasayana (Arogyavardhini Rasa)

Background: Arogyavardhini Rasa is a well known Ayurvedic herbo-mineral formulation widely used for a broad spectrum of therapeutic indications mainly in the treatment of Kamala (jaundice) and other Yakrit Vikara (liver disorders). Aim: To standardized Arogyavardhini Rasa prepared with and without Rasavarga Dravya (ingredients of Metallo-mineral origin) by using high-performance thin-layer chromatography (HPTLC)  & computational by principal component analysis (PCA)  as an analytical tool.    Materials & Method: Arogyavardhani Rasa (AVR) was prepared as per classical reference and further modified this preparation by omitting  Rasavarga dravya (ingredients of Metallo-mineral origin) of Arogyavardhani Rasa (MAVR). Formulations were investigated for physicho-chemical parameters, chromatography and using the building block of chemometrics technique such as PCA was applied to discriminate the formulations AVR and MAVR. Result & Discussion: HPTLC study revealed a total of 11 and 8 bands at 254 nm and 366 nm in both AVR & MAVR, with only two similar Rf values in the sample. PCA revealed that both formulations were found similarities & dissimilarities within a batch of AVR and MAVR with its physicho-chemical constant. Conclusion: The analytical tools applied (HPTLC and PCA)  for the study revealed that the classical processing like Bhavana (Lavigation ), Mardana (Trituration) etc. had a significant role in the preparation of Rasaaushadhis (mercurial medicine) and process validate the Indian system of medicinal pharmaceutics.