{"title":"芭蕉叶束顶病毒PCR检测的高效DNA提取方法","authors":"S. Johari, S. Majumder","doi":"10.3923/AJBKR.2015.80.87","DOIUrl":null,"url":null,"abstract":"Banana bunchy top virus (BBVT) is the most threatening disease of banana all over the world. Control of BBTV involves the use of disease free planting material and prevention of infection. Its accurate detection is one of the functional approach to control it by eradication and exclusion. PCR is the most efficient mode of indexing but high-quality of DNA is one of the most essential factor for a successful amplification by PCR. In this study, we have developed a highly efficient low cost and rapid DNA extraction method for banana tissue which could yield good quantity of DNA free from protein and polysaccharide contamination. The DNA extracted by this method could be successfully amplified by PCR. In addition, the protocol could be completed within ~2 h.","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"An Efficient DNA Extraction Protocol for Successful PCR Detection of Banana bunchy top virus from Banana Leaves\",\"authors\":\"S. Johari, S. Majumder\",\"doi\":\"10.3923/AJBKR.2015.80.87\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Banana bunchy top virus (BBVT) is the most threatening disease of banana all over the world. Control of BBTV involves the use of disease free planting material and prevention of infection. Its accurate detection is one of the functional approach to control it by eradication and exclusion. PCR is the most efficient mode of indexing but high-quality of DNA is one of the most essential factor for a successful amplification by PCR. In this study, we have developed a highly efficient low cost and rapid DNA extraction method for banana tissue which could yield good quantity of DNA free from protein and polysaccharide contamination. The DNA extracted by this method could be successfully amplified by PCR. In addition, the protocol could be completed within ~2 h.\",\"PeriodicalId\":274901,\"journal\":{\"name\":\"Asian Journal of Biotechnology\",\"volume\":\"26 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Asian Journal of Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3923/AJBKR.2015.80.87\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Asian Journal of Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3923/AJBKR.2015.80.87","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
An Efficient DNA Extraction Protocol for Successful PCR Detection of Banana bunchy top virus from Banana Leaves
Banana bunchy top virus (BBVT) is the most threatening disease of banana all over the world. Control of BBTV involves the use of disease free planting material and prevention of infection. Its accurate detection is one of the functional approach to control it by eradication and exclusion. PCR is the most efficient mode of indexing but high-quality of DNA is one of the most essential factor for a successful amplification by PCR. In this study, we have developed a highly efficient low cost and rapid DNA extraction method for banana tissue which could yield good quantity of DNA free from protein and polysaccharide contamination. The DNA extracted by this method could be successfully amplified by PCR. In addition, the protocol could be completed within ~2 h.
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