{"title":"乳酸菌基因工程与培养的方向与方法供初学者参考","authors":"K. Okano","doi":"10.4109/jslab.30.8","DOIUrl":null,"url":null,"abstract":"Genetic engineering of lactic acid bacteria is a difficult task for the beginners due to large difference in strains of interest and poor access for genetic tools. In this review, based on the author’ s experiences, experimental directions and methods for selecting the host strain and vectors; transformation of lactic acid bacteria; optimization of expression system; gene disruption/insertion; cultivation of recombinants","PeriodicalId":117947,"journal":{"name":"Japanese Journal of Lactic Acid Bacteria","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The directions and methods of genetic engineering and cultivation of lactic acid bacteria for the beginners\",\"authors\":\"K. Okano\",\"doi\":\"10.4109/jslab.30.8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Genetic engineering of lactic acid bacteria is a difficult task for the beginners due to large difference in strains of interest and poor access for genetic tools. In this review, based on the author’ s experiences, experimental directions and methods for selecting the host strain and vectors; transformation of lactic acid bacteria; optimization of expression system; gene disruption/insertion; cultivation of recombinants\",\"PeriodicalId\":117947,\"journal\":{\"name\":\"Japanese Journal of Lactic Acid Bacteria\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-03-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese Journal of Lactic Acid Bacteria\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4109/jslab.30.8\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Lactic Acid Bacteria","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4109/jslab.30.8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The directions and methods of genetic engineering and cultivation of lactic acid bacteria for the beginners
Genetic engineering of lactic acid bacteria is a difficult task for the beginners due to large difference in strains of interest and poor access for genetic tools. In this review, based on the author’ s experiences, experimental directions and methods for selecting the host strain and vectors; transformation of lactic acid bacteria; optimization of expression system; gene disruption/insertion; cultivation of recombinants
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