用薄荷(Mentha piperita)和甘蓝(Brassica oleracea var. capitata)提取物水溶液合成绿色纳米银及其抗菌活性

M. Sosnowska, Marta Kutwin, A. Adamiak, K. Gawin, Ż. Bugajska, Karolina Daniluk
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引用次数: 4

摘要

用薄荷(Mentha piperita)和甘蓝(Brassica oleracea var. capitata)提取物水溶液合成绿色纳米银及其抗菌活性。本研究的目的是用薄荷叶和卷心菜提取物作为还原剂和稳定剂合成纳米银(Ag-NP)。纳米颗粒的存在首先通过获得的颜色确认,然后通过透射电子显微镜(TEM)确认。所得Ag-NP的TEM分析表明,薄荷的Ag-NP大小为5 ~ 50 nm,白菜的Ag-NP大小为10 ~ 150 nm。采用PrestoBlue法和XTT法测定纳米颗粒对病原菌大肠杆菌、金黄色葡萄球菌和肠炎沙门氏菌的抑菌活性。与革兰氏阳性菌(金黄色葡萄球菌)相比,革兰氏阴性菌(大肠杆菌、肠链球菌)对细菌活力的抑制作用更高。
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Green synthesis of silver nanoparticles by using aqueous mint (Mentha piperita) and cabbage (Brassica oleracea var. capitata) extracts and their antibacterial activity
Green synthesis of silver nanoparticles by using aqueous mint (Mentha piperita) and cabbage (Brassica oleracea var. capitata) extracts and their antibacterial activity. The objective of this study was the synthesis of silver nanoparticles (Ag-NP) using leaves of mint and cabbage extracts as the reducing and stabilising agents. The presence of nanoparticles was initially confirmed by the obtained colour and next by transmission electron microscope (TEM). Analysis of TEM of obtained Ag-NP indicated that their size ranged 5–50 nm for mint and 10–150 nm for cabbage. The antibacterial activity of nanoparticles against pathogenic strains Escherichia coli, Staphylococcus aureus and Salmonella enterica were assessed by evaluation of metabolic activity, using the PrestoBlue and XTT test. The higher inhibition of bacterial viability was observed against Gram-negative (E. coli, S. enterica) than Gram-positive (S. aureus) bacteria.
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