自旋俘获ESR法检测紫外透镜中自由基。

Lens and eye toxicity research Pub Date : 1992-01-01

J Murakami, Y Kozuka, M Okazaki, T Shiga

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引用次数: 0

摘要

我们对晶状体的紫外光解进行了ESR研究，并利用自旋俘获技术确定了参与初始光化学过程的自由基的来源。在自旋捕获试剂(DMPO)的作用下，对犬晶状体进行了两种自旋加合物的检测;由谷胱甘肽衍生的以硫为中心的自由基的自旋加合物和水合电子的质子化加合物。通过与色氨酸+半胱氨酸溶液光解作用的比较，探讨了紫外线照射晶状体初始光化学损伤的自由基机制。

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Detection of free radicals in UV-irradiated lens by spin trapping ESR.

We have made an ESR study on the UV-photolysis of lens and identified the origins of free radicals involved in the initial photochemical process by spin trapping technique. Two spin adducts were detected on irradiation of canine lens in the presence of a spin trapping reagent (DMPO); a spin adduct of sulfur centered radical derived from glutathione and the protonated adduct of hydrated electron. A free radical mechanism of initial photochemical injury in UV-irradiated lens was discussed, comparing with a photolysis of tryptophan plus cysteine solution.

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Lens and eye toxicity research

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