林木种子的低温保存研究进展

S. Gantait, Suprabuddha Kundu, S. H. Wani, P. K. Das
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引用次数: 9

摘要

森林树木是森林生态系统的基础,对各种动植物的生存至关重要。维持森林生态系统的首要挑战包括森林树木数量的减少以及由于造林和森林面积开发、环境变化、害虫、污染和渐渗杂交而引起的结构变化。对于迁地保护方法,体外技术在保护树木遗传资源方面发挥了基本作用,特别是在自然繁殖体如顽固性种子可能不适合长期保护的情况下。这种做法包括限制生长技术、传统的微繁殖、合成种子的生产和储存以及冷冻保存。尽管这些做法主要应用于草本物种,但最近,木本物种也受到关注。本文简要讨论了林木种子保存的关键概念、挑战和技术,重点介绍了一些成功的长期冷冻保存方法。
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Cryopreservation of Forest Tree Seeds: A Mini-Review
Since forest trees form the basis of forest ecosystem, their prolong subsistence is crucial for various flora and fauna. The foremost challenges to sustain the forest ecosystem comprise of the declining forest tree population accompanied with structural changes due to afforestation and exploitation of forest area, environment changes, pests, pollution, and introgressive hybridization. For ex situ conservation approach, in vitro techniques encompass basic role for conserving tree genetic resources, predominantly where natural propagules like recalcitrant seed might not be appropriate for long-term conservation. The practice includes restricted growth techniques, conventional micropropagation, production and storage of synthetic seeds, and cryopreservation. Even though these practices have been applied chiefly to herbaceous species, but recently, woody species were also focused upon. Key conceptions, challenges and techniques for forest tree seed conservation are discussed briefly in this review with special emphasis on some successful cryopreservation approaches for long-term storage.
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