提高DHM分辨率的不同方法

A. Faridian, D. Hopp, G. Pedrini, W. Osten, U. Eigenthaler, M. Hirscher
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引用次数: 0

摘要

提出了提高数字全息显微镜(DHM)分辨率的不同方法。在离轴深紫外DHM装置中实现了一种高分辨率的方法,使用倾斜照明克服了光学系统引入的限制,并通过实验验证了250 nm的理论分辨率极限(根据阿贝准则)。为了提高复杂生物样品三维成像的分辨率和对比度,还介绍了一种工作在可见光波段的双视图DHM系统。
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Different approaches to increase resolution in DHM
Different approaches to increase the resolution in digital holographic microscopy (DHM) are presented. A high resolution approach has been implemented in the off axis Deep UV DHM setup using oblique illumination to overcome the limitation introduced by the optical system and the theoretical resolution limit of 250 nm (according to Abbe criterion) has been verified, experimentally. A two view DHM system operating in the visible wavelength is also introduced to increase the resolution and contrast in 3D imaging of complex biological samples.
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