Raymond E. Gibson , H.Donald Burns , Holly H. Thorpe , Wai-Si Eng , Richard Ransom , Howard Solomon
{"title":"(+)−3-[125I]碘- mk -801在大鼠脑内与NMDA受体通道复合物的体内结合和放射自显像","authors":"Raymond E. Gibson , H.Donald Burns , Holly H. Thorpe , Wai-Si Eng , Richard Ransom , Howard Solomon","doi":"10.1016/0883-2897(92)90117-H","DOIUrl":null,"url":null,"abstract":"<div><p>Radioiodinated (+)−3-Iodo-MK-801 is a high affinity radioligand for the <em>N</em>-methyl-<span>d</span>-aspartate (NMDA) receptor-channel complex. We have demonstrated <em>in vivo</em> localization in the CNS of rat which is stereoselective and blocked by coinjection of unlabeled MK-801. Autoradiography indicates localization <em>in vivo</em> which is in concordance with <em>in vitro</em> autoradiographic studies. These results indicate that radioiodinated (+)−3-Iodo-MK-801 is a useful probe for <em>in vitro</em> and <em>in vivo</em> autoradiographic studies and suggest that radioligands for the NMDA receptor may be developed which will provide <em>in vivo</em> images of receptor distribution in man.</p></div>","PeriodicalId":14328,"journal":{"name":"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology","volume":"19 3","pages":"Pages 319-326"},"PeriodicalIF":0.0000,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0883-2897(92)90117-H","citationCount":"17","resultStr":"{\"title\":\"In vivo binding and autoradiographic imaging of (+)−3-[125I]Iodo-MK-801 to the NMDA receptor-channel complex in rat brain\",\"authors\":\"Raymond E. Gibson , H.Donald Burns , Holly H. Thorpe , Wai-Si Eng , Richard Ransom , Howard Solomon\",\"doi\":\"10.1016/0883-2897(92)90117-H\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Radioiodinated (+)−3-Iodo-MK-801 is a high affinity radioligand for the <em>N</em>-methyl-<span>d</span>-aspartate (NMDA) receptor-channel complex. We have demonstrated <em>in vivo</em> localization in the CNS of rat which is stereoselective and blocked by coinjection of unlabeled MK-801. Autoradiography indicates localization <em>in vivo</em> which is in concordance with <em>in vitro</em> autoradiographic studies. These results indicate that radioiodinated (+)−3-Iodo-MK-801 is a useful probe for <em>in vitro</em> and <em>in vivo</em> autoradiographic studies and suggest that radioligands for the NMDA receptor may be developed which will provide <em>in vivo</em> images of receptor distribution in man.</p></div>\",\"PeriodicalId\":14328,\"journal\":{\"name\":\"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology\",\"volume\":\"19 3\",\"pages\":\"Pages 319-326\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0883-2897(92)90117-H\",\"citationCount\":\"17\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/088328979290117H\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/088328979290117H","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
In vivo binding and autoradiographic imaging of (+)−3-[125I]Iodo-MK-801 to the NMDA receptor-channel complex in rat brain
Radioiodinated (+)−3-Iodo-MK-801 is a high affinity radioligand for the N-methyl-d-aspartate (NMDA) receptor-channel complex. We have demonstrated in vivo localization in the CNS of rat which is stereoselective and blocked by coinjection of unlabeled MK-801. Autoradiography indicates localization in vivo which is in concordance with in vitro autoradiographic studies. These results indicate that radioiodinated (+)−3-Iodo-MK-801 is a useful probe for in vitro and in vivo autoradiographic studies and suggest that radioligands for the NMDA receptor may be developed which will provide in vivo images of receptor distribution in man.
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