Insecticide spray training at Shinjuku Gyoen National Garden in preparation for autochthonous dengue fever

To prepare for an epidemic of mosquito-borne diseases including dengue, chikungunya and Zika, we demonstrated a training of insecticide spray to eliminate Aedes albopictus at Shinjuku Gyoen National Garden. ree insecticide-spraying methods were used and treatment e cacy of each method was evaluated; 302.5 m2/min (carbon dioxide formulation), 69.4 m2/min (ultra low volume, ULV), and 71.2 m2/min (power spray). It was shown that it is important to select the most appropriate insecticide application method and formulation depending on the size of the eld, available number of workers, and the weather conditions.