改进共聚焦显微镜对半透明介质表面的检测

Peter R. Nyholm, Jared E. Payne, Robert W. Lucas, J. D. Jensen, Ryan T. Beazer, Kevin E. Larson, G. Nielson, S. Schultz
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引用次数: 0

摘要

提出了一种改进的共聚焦显微镜系统,将激光束的焦点放在半透明介质的表面。被检测表面的分辨率显示为依赖于针孔大小和在共聚焦系统中使用的显微镜物镜的数值孔径。使用20µm针孔和0.25数值孔径共聚焦透镜演示了蓝宝石窗口前后表面的检测。
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Surface Detection of Semi-Transparent Media Through Modified Confocal Microscopy
A modified confocal microscope system is developed to place the focal point of a laser beam on the surface of semitransparent media. Resolution of the detected surfaces is shown to be dependent on pinhole size and numerical aperture of the microscope objective used in the confocal system. Detection of front and back surfaces of a sapphire window is demonstrated using a 20 µm pinhole and 0.25 numerical aperture confocal focusing lens.
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