胡杨抗真菌生物化合物的色谱诊断

R. Khaleel
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引用次数: 3

摘要

在生物测定试验中,从原料中筛选植物提取物是确定新的生物活性、毒性和活性化合物的必要条件。摘要对胡杨生叶进行了植物化学筛选、成分筛选、分离鉴定和MIC抑菌试验。采用液相色谱-质谱联用-质谱联用进行生物化合物分析。粗提物的植物化学筛选表明,与其他化合物相比,存在糖苷。乙醇提取物对真菌的抑制作用达到390,mg/L/MIC。采用对虾致死试验(LC50)进行生物毒性测定,结果显示4.1 mg/L为低毒,可安全使用。α糖苷(4,6苄基- 10 -甲基- 20 -(2346 - 4 - o -乙酰- β -葡萄糖基)是一种具有抗真菌作用的新化合物。
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Diagnosis of the Bio-Compound as an Anti-Fungal from Populus euphratica L. Plant Using Chromatographic Technology
Crude of Plant extracts from materials is necessary screening to determine new biological activities, toxicity and active compounds in bioassay tests. Crude of Populus euphratica L. left is tested to phytochemical screening, constituents, fractionation and MIC technique as anti-fungal. The fractions are subjected to bio-compound analysis using Liquid chromatography-mass spectrometry LC-MS. Phytochemical screening of crude extract shows presence of Glycosides compared with other compounds. While the fraction of ethanolic extract recorded inhibition impact reached (390, mg/L/MIC) agnist type of fungi. Bioassay toxicity using shrimp lethally test (LC50) was carried out with the result showing 4.1 mg/L is low toxic and can be used safely. Alpha Glycoside (4, 6 Benzylidene-1O-Methyl-2O-(2346 Tetra-O-Acetyl-Betad- Glucosyl), is a new compound and antifungal action.
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