Comparative assessment of prostaglandin E2 levels in gingival crevicular fluid after diode laser pocket therapy: A clinico-biochemical study

Introduction: Conventional periodontal therapy in form of scaling and root planing (SRP) has its own limitations when used in deep pockets. Various studies have reported adjunctive use of laser therapy as a promising modality to overcome limitations of SRP. Aim and Objectives of the Study: The aim of this study was to assess the adjunctive effect of 810-nm diode laser on clinical parameters and prostaglandin E2 (PGE2) levels in gingival crevicular fluid (GCF). Materials and Methods: The present study was a split-mouth clinical study, which included 48 sites from patients diagnosed with moderate-to-severe chronic periodontitis. Test group consisted of 24 sites that received diode laser (810 nm, 2.5 W) therapy as an adjunct to SRP whereas control group consisted of 24 sites, which received SRP only. Gingival index (GI) was assessed clinically whereas Florida Probe was used to record bleeding on probing (BOP), pocket probing depth (PPD), and clinical attachment level (CAL) at baseline, 3 months, and 6 months. GCF PGE2levels were measured at baseline and 3 months post therapy using commercially available ELISA kit (Neogen™). Results: Test group showed a significant reduction in GCF PGE2levels at the end of 3 months when compared to control sites. However, the primary clinical parameters (CAL and PPD) from baseline to 3 and 6 months did not show statistically significant improvement in the test group. Conclusion: Application of diode laser resulted in a significant reduction in PGE2levels compared to SRP alone. Thus, diode laser therapy as an adjunct to SRP appears to be a promising treatment modality to reduce or modify the host response.