电穿孔法转染大肠杆菌lambda DNA。

Microbiologica Pub Date : 1992-10-01

C Magistrelli, E Colombo, A Tognoni, G Grandi

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引用次数: 0

摘要

在枯草芽孢杆菌基因组测序和定位项目的范围内，我们建立了用lambda DNA转染大肠杆菌细胞的电穿孔方法。这种方法提出的特点，使其优于传统的体外包装的某些目的。在这里，我们将说明实验过程和可能的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Transfection of E. coli with lambda DNA by electroporation.

In the ambit of the B. subtilis genoma sequencing and mapping project, we have set up an electroporation method to transfect E. coli cells with lambda DNA. This methodology presents features that make it preferable to traditional in vitro packaging for some purposes. Here we will illustrate the experimental procedure and the possible applications.

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Microbiologica

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