PENGEMBANGAN DAN VALIDASI METODE SPEKTROFOTOMETRI UV VIS METODE DERIVATIF UNTUK ANALISIS KAFEIN DALAM SUPLEMEN

Supplements are health products that are consumed to meet the body's health needs. Usually shaped in dosage tablets or powder forms, it contains active ingredients, such as caffeine. In 2004, POM issued a decree of No. HK.00.05.23.3644 concerning 'Basic Provisions for Supervision of Food Supplements.' This decision states that the maximum limit of daily caffeine intake is 150 mg/day, and more than 150 mg/day consumption could cause side effects. In this study, an experimental study was conducted to validate and develop the analysis of the method using UV-Vis spectrophotometry using the derivative method to analyze the caffeine in supplements. In this research, optimization of solvent from four categories has been done, and methanol was selected as the solvent with the mixture of citric acid, and then carried out using a derivative method with dA/d by using a maximum wavelength of 239 nm because in this analysis there was a wavelength shift. The parameters for selectivity, linearity, precision, and accuracy were utilized to prove the validity of the method. After the validity was declared, the determination of caffeine content in the supplement was performed. The result from caffeine analysis with spectrophotometry UV-Vis with derivatives method show a result of coefficient of determination (R2) 0.988; coefficient of correlation (r) 0.976 with pvalue 5,46 x 10^-5; limit of detection 4,5ppm; limit of quantification 13,6ppm; and degree of deviation 3.750% with an accuracy of 97.443%. These results conclude that the method was valid. This method can be used for caffeine analysis.