{"title":"用酶联免疫吸附法测定新城疫病毒抗体。","authors":"V A Srinivasan, G S Reddy, A P Kalanidhi","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Serum antibody assay of 1045 serum samples from birds vaccinated with Newcastle Disease vaccine by HI test and ELISA was carried out. Five hundred and twenty seven birds were challenged with virulent virus and data on HI test, ELISA and challenge test results were used for comparison. A good correlation between HI titres, ELISA absorbance and potency was observed indicating the usefulness of ELISA for potency estimation of vaccines and determination of immunization level in vaccinated flocks.</p>","PeriodicalId":77264,"journal":{"name":"Microbiologica","volume":"15 3","pages":"319-21"},"PeriodicalIF":0.0000,"publicationDate":"1992-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Use of enzyme linked immunosorbent assay for estimation of antibody to Newcastle disease virus.\",\"authors\":\"V A Srinivasan, G S Reddy, A P Kalanidhi\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Serum antibody assay of 1045 serum samples from birds vaccinated with Newcastle Disease vaccine by HI test and ELISA was carried out. Five hundred and twenty seven birds were challenged with virulent virus and data on HI test, ELISA and challenge test results were used for comparison. A good correlation between HI titres, ELISA absorbance and potency was observed indicating the usefulness of ELISA for potency estimation of vaccines and determination of immunization level in vaccinated flocks.</p>\",\"PeriodicalId\":77264,\"journal\":{\"name\":\"Microbiologica\",\"volume\":\"15 3\",\"pages\":\"319-21\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiologica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiologica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Use of enzyme linked immunosorbent assay for estimation of antibody to Newcastle disease virus.
Serum antibody assay of 1045 serum samples from birds vaccinated with Newcastle Disease vaccine by HI test and ELISA was carried out. Five hundred and twenty seven birds were challenged with virulent virus and data on HI test, ELISA and challenge test results were used for comparison. A good correlation between HI titres, ELISA absorbance and potency was observed indicating the usefulness of ELISA for potency estimation of vaccines and determination of immunization level in vaccinated flocks.
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