Immunization with Hpv16e7d Vaccine to Tumor Bearing Mice: Changes of Cytokines Patters in the Tumor Microenvironment

Immune response patter in the tumor environment determines the outcome of tumor prognosis. Here, tumor-bearing mice were immunized with HPV17E7d vaccine at a distant area of the tumor and balance of cytokines were assessed in the tumor microenvironment. Tumor was established in C57BL/6 mice and mice were vaccinated subcutaneously, three times with two weeks intervals with 20 µg of E7d protein. Two weeks after final immunization, tumor homogenate was prepared and the quantity of IL-2, IL-12, IL-17 and TNF-α cytokines were evaluated with commercial ELISA kits. In addition, lymphocyte proliferation of spleen cells was determined with BRDU method. Results show that immunization with vaccine candidates increased IL-2, IL-12, IL-17 and TNF-α cytokines and lymphocyte proliferation versus un-vaccinated PBS control group. As regard to results of cytokines and lymphocyte proliferation could conclude that tumor microenvironment has been modified by changing cytokines patterns to defeat tumor cell using tumor vaccination even at a distant area of the tumor.