James F. Kronauge , Mary L. Chiu , Jeffrey S. Cone , Alan Davison , B.Leonard Holman , Alun G. Jones , David Piwnica-Worms
{"title":"中性和阳离子心肌灌注剂的比较:培养细胞的蓄积特征","authors":"James F. Kronauge , Mary L. Chiu , Jeffrey S. Cone , Alan Davison , B.Leonard Holman , Alun G. Jones , David Piwnica-Worms","doi":"10.1016/0883-2897(92)90001-F","DOIUrl":null,"url":null,"abstract":"<div><p>Uptake and washout kinetics of two new neutral lipophilic technetium-99m-labeled boronic acid adducts of technetium tris(dioxime) (BATO complexes) were studied in monolayers of contractile chick heart cells and compared to the cationic myocardial perfusion agents, <sup>99m</sup>Tc(CNCH<sub>2</sub>C(CH<sub>3</sub>)<sub>2</sub>OCH<sub>3</sub>)<sup>+</sup><sub>6</sub> (Tc-MIBI) and <sup>201</sup>Tl<sup>+</sup>. <sup>99m</sup>TcCl(CDOH)<sub>2</sub>(CDO)(BCH<sub>3</sub>), where CDO = cyclohexanedione dioxime (CDO-MeB), had a 7-fold greater net accumulation than Tc-MIBI and the most rapid unidirectional washout with a fast initial phase and a slower secondary component. Incubation with cationic membrane transport inhibitors or metabolic inhibitors had little or modest influence, respectively, on uptake of these BATO complexes. Studies with NIH 3T3 fibroblasts indicated that the neutral complexes did not show myocyte specific accumulation.</p></div>","PeriodicalId":14328,"journal":{"name":"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology","volume":"19 2","pages":"Pages 141-148"},"PeriodicalIF":0.0000,"publicationDate":"1992-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0883-2897(92)90001-F","citationCount":"35","resultStr":"{\"title\":\"Comparison of neutral and cationic myocardial perfusion agents: Characteristics of accumulation in cultured cells\",\"authors\":\"James F. Kronauge , Mary L. Chiu , Jeffrey S. Cone , Alan Davison , B.Leonard Holman , Alun G. Jones , David Piwnica-Worms\",\"doi\":\"10.1016/0883-2897(92)90001-F\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Uptake and washout kinetics of two new neutral lipophilic technetium-99m-labeled boronic acid adducts of technetium tris(dioxime) (BATO complexes) were studied in monolayers of contractile chick heart cells and compared to the cationic myocardial perfusion agents, <sup>99m</sup>Tc(CNCH<sub>2</sub>C(CH<sub>3</sub>)<sub>2</sub>OCH<sub>3</sub>)<sup>+</sup><sub>6</sub> (Tc-MIBI) and <sup>201</sup>Tl<sup>+</sup>. <sup>99m</sup>TcCl(CDOH)<sub>2</sub>(CDO)(BCH<sub>3</sub>), where CDO = cyclohexanedione dioxime (CDO-MeB), had a 7-fold greater net accumulation than Tc-MIBI and the most rapid unidirectional washout with a fast initial phase and a slower secondary component. Incubation with cationic membrane transport inhibitors or metabolic inhibitors had little or modest influence, respectively, on uptake of these BATO complexes. Studies with NIH 3T3 fibroblasts indicated that the neutral complexes did not show myocyte specific accumulation.</p></div>\",\"PeriodicalId\":14328,\"journal\":{\"name\":\"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology\",\"volume\":\"19 2\",\"pages\":\"Pages 141-148\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0883-2897(92)90001-F\",\"citationCount\":\"35\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/088328979290001F\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/088328979290001F","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Comparison of neutral and cationic myocardial perfusion agents: Characteristics of accumulation in cultured cells
Uptake and washout kinetics of two new neutral lipophilic technetium-99m-labeled boronic acid adducts of technetium tris(dioxime) (BATO complexes) were studied in monolayers of contractile chick heart cells and compared to the cationic myocardial perfusion agents, 99mTc(CNCH2C(CH3)2OCH3)+6 (Tc-MIBI) and 201Tl+. 99mTcCl(CDOH)2(CDO)(BCH3), where CDO = cyclohexanedione dioxime (CDO-MeB), had a 7-fold greater net accumulation than Tc-MIBI and the most rapid unidirectional washout with a fast initial phase and a slower secondary component. Incubation with cationic membrane transport inhibitors or metabolic inhibitors had little or modest influence, respectively, on uptake of these BATO complexes. Studies with NIH 3T3 fibroblasts indicated that the neutral complexes did not show myocyte specific accumulation.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
