Analytical Studies and Biological Activity of New Azo Dye and Its Complexation with Zinc

Azo dye (AD), (Z)-5-amino-2-(((4-(5-(p-tolyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl) phenyl) sulfonyl) diazenyl) phenol) and its complex with zinc were prepared and characterized using FTIR, UV-visible, elemental analysis and mass spectrum. Analytical studies showed that this dye had high solubility in ethanol and gave three isopiestic points when studying the pH effect in a range of buffer solutions. The protonation constants pKb1 and pKb2 of nitrogen atom and the ionization constant pKa of OH-group were equal to 3.4, 7.26 and 10.5 respectively. These results indicated the suggested ionization scheme in acidic and basic media. Further, the synthetic AD was provided non-toxic effects using different concentrations, and didn't show any heamolysis effects in the cells in contrast with another chemical compound. The new AD is considered as novel medicine or chemical sanitizer. Further, the biological activity of AD and its complex was tested against four different bacteria, (Staphylococcus Aureus, Escherichia Coli, Bacillus Cereus and Pseudomonas Aeruginosa) and two different fungi (Aspergillus Albicans and Candida Albicans). The results showed that the complexation of AD with zinc was decreased the biological activity of synthetic AD.