离子交换色谱法从动物血液中提取白蛋白的方法研究

G. Leaver, J. Conder, J. Howell
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引用次数: 7

摘要

摘要:研究了用离子交换色谱法从牛血清和血浆中提取白蛋白的操作条件。离子交换剂是Vistec-DEAE纤维素。在各种可用的色谱操作模式中,最适合蛋白质的是在整个色谱柱上吸附,然后逐步洗脱。该方法是通过研究发展起来的;实验分析了主要参数的影响,从而为优化指明了方向。预先透析和稀释饲料是有益的。其他结论一般适用于蛋白质的大规模分离。特别是,柱长,洗脱液速度,进料带宽度,和填料刚性是参数,权衡将需要最大限度地提高性能。如果在柱设计中采取适当的预防措施,可以通过单独增加柱直径来实现放大。用色谱法分离的白蛋白在电泳纯度上优于商用Cohn沉淀法。
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A Method Development Study of the Production of Albumin from Animal Blood by Ion-Exchange Chromatography
Abstract The conditions of operation of ion-exchange Chromatography are examined for the production of albumin from bovine serum and plasma. The ion-exchanger is a Vistec-DEAE cellulose. Of the various modes of chromatographic operation available, the most suitable for proteins is shown to be adsorption onto the whole column followed by stepwise elution. The method is developed by studying; experimentally, the effect of major parameters so as to point the way to optimization. Prior dialysis and dilution of the feed are beneficial. Other conclusions apply to the large-scale separation of proteins in general. In particular, column length, eluent velocity, feed band width, and packing rigidity are parameters where trade-offs will be needed to maximize performance. If suitable precautions are taken in column design, scale-up can be achieved by increasing the column diameter alone. Albumin separated chromatographically is superior in electrophoretic purity to that produced by the commercial Cohn precipitation ...
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