一种与雄性鳞叔翅虫减数分裂相关的细胞表面抗原

Melanie J. Percy, Hugh L. Fletcher, Alan Trudgett
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引用次数: 0

摘要

免疫荧光已鉴定出7种与减数分裂细胞表面反应而在减数分裂前细胞中不存在的单克隆抗体。免疫金电镜分析表明,这些抗原存在于细胞外表面,与细胞核内突触复合物的存在一致。在免疫印迹上,除了较小的亚基外,还识别出205 kDa的常见糖基化蛋白,这表明存在由较小肽组成的蛋白质复合物。
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A cell surface antigen associated with meiosis in male Staurdoderus scalaris(orthopteran)

Immunofluorescence has identified seven monoclonal antibodies reactive with the surface of meiotic cells and absent in premeiotic cells. Analysis by immunogold electron microscopy indicated that these antigens were present on the external surface of the cells and were coincident with the presence of synaptonemal complexes in the nucleus. On immunoblots a common glycosylated protein of 205 kDa was recognized, in addition to smaller subunits, suggesting the presence of a protein complex comprised of smaller peptides.

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