W. Wijanarka, Sri Pujiyanto, Budi Raharjo
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摘要

葡聚糖酸细菌是一种能够将葡聚糖分解成葡萄糖单体的细菌。细菌的这种能力是由葡聚糖酶的存在引起的。从细菌中提取葡聚糖酶的选择是基于细菌在生产葡聚糖酶方面的生长能力和速度。消化细菌和原生动物在消化道中相互共生以消化纤维素或伴生物质根据蜗牛的生活方式消化牧草和凋落叶的能力,人们怀疑蜗牛(Achatina fulica)具有生产葡聚糖酶生物催化剂的能力,特别是在消化道中。为了解螺管土生菌的特性和特点,对KE-B6菌进行分子鉴定,得到准确准确的结果。基于16S rDNA序列数据,用27F(正向)和1492R(反向)引物对KE-B6分离菌进行BLAST比对,结果显示菌株与粘质沙雷氏菌同源性为99.64%。关键词:细菌,葡聚糖,黄芽孢杆菌,粘质沙雷氏菌
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Identifikasi Molekular Bakteri Glukanolitik Indigenous KE-B6 dari Saluran Pencernaan Bekicot (Achatina fulica)
Glucanolitic bacteria are bacteria that have the ability to break down glucan into glucose monomer units. The ability of the bacteria is caused by the presence of glucanase enzymes. The choice of glucanase derived from bacteria is based on the ability and speed of bacterial growth in terms of producing glucanase enzymes. The presence of bacteria and protozoa in the digestive tract symbiotic with each other to digest cellulose or concomitant materials . Based on the ability of the way of life to digest forage and leaf litter, it is suspected that snails (Achatina fulica) have the ability to produce glucanase biocatalysts, especially in the digestive tract. To find out the characteristics and characteristics of indigenous bacteria snail canals, identification of KE-B6 bacteria is carried out molecularly so that accurate and accurate results are obtained. The Basic Alignment Search Tools BLAST results of KE-B6 bacterial isolates based on 16S rDNA sequence data with 27F (Forward) and 1492R (Reverse) primers showed that these bacterial isolates had homology of 99.64% to Serratia marcescens.  Key words: bacteria, glucanolitic, A. fulica, Serratia marcescens.
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