黄斑红斑白斑突变体对黄斑白斑毒株的适应性和生长反应

Muhklisani, P. Karti, I. Prihantoro
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摘要

在组织培养技术中,驯化是植物繁殖的最后阶段,决定苗圃过程的成功与否。本研究旨在观察组织培养技术培育的银合欢(Leucaena leucocephala Tarramba)品种在酸适应期的生长响应。本研究采用完全随机设计(CRD),从组织培养中培养11个突变系,即M1-M11(从组织培养中添加1ppm IBA获得的酸适应突变体)和2个对照(没有γ辐照的lamtoro亲鱼),即K0(组织培养中添加0 ppm IBA获得的lamtoro亲鱼),K1(从组织培养中添加1ppm IBA育种lamtoro)。观察到的变量是植株活力水平、株高和叶片数。结果表明,在M3和M9突变系上,白头银合子对组培产量的驯化在种植后5wap(周)内对植株形态生长的响应最好。关键词:驯化,IBA激素,银合欢,组织培养
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Aklimatisasi dan Respon Pertumbuhan Mutan Leucaena Leucocephala Varietas Tarramba Teradaptasi Asam
Acclimatization is the final stage of plant propagation in tissue culture techniques that can determine the success of the nursery process. This study aimed to observe the growth response during the acclimatization stage of the acid-adapted of Leucaena leucocephala Tarramba variety, which developed from tissue culture techniques. The design used in this study was a completely randomized design (CRD) with the cultivation of 11 mutant lines from tissue culture, namely M1-M11 (an acid-adapted mutant from tissue culture addition of 1 ppm IBA) and 2 controls (lamtoro broodstock without gamma irradiation) namely K0 (lamtoro broodstock resulting from tissue culture addition of 0 ppm IBA), K1 (breeding lamtoro from tissue culture addition of 1 ppm IBA). The variables observed were the level of plant viability, plant height, and number of leaves. The results showed that the acclimatization of the plant Leucaena leucocephala to tissue culture production on the M3 and M9 mutant lines gave the best response to plant morphological growth up to 5 WAP (weeks after planting). Key words:        acclimatization, IBA hormone, Leucaena leucocephala, tissue culture
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