{"title":"从FTMS测量抑制肽谱的迭代校正","authors":"Xuepo Ma, T. Hestilow, Jian Cui, Jianqiu Zhang","doi":"10.1109/BIBM.2010.5706567","DOIUrl":null,"url":null,"abstract":"Analysis of peptide profiles from a Liquid Chromatography Fourier Transform Mass Spectrometry (LC/FTMS) measurement reveals a non-linear distortion in intensity. Investigation of the measured CVi /C12 ratios comparing with theoretical ones shows that the non-linearity can be attributed to low intensity signal suppression of low abundance peptide peaks. We find that the suppression is homogenous for different isotopes of identical peptides but non-homogenous for different peptides. We developed an iterative correction algorithm that corrects the intensity distortions for peptides with relatively high abundance. This algorithm can be applied in a wide range of applications using FTMS.","PeriodicalId":275098,"journal":{"name":"2010 IEEE International Conference on Bioinformatics and Biomedicine (BIBM)","volume":"82 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Iterative correction of suppressed peptide profiles from FTMS measurements\",\"authors\":\"Xuepo Ma, T. Hestilow, Jian Cui, Jianqiu Zhang\",\"doi\":\"10.1109/BIBM.2010.5706567\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Analysis of peptide profiles from a Liquid Chromatography Fourier Transform Mass Spectrometry (LC/FTMS) measurement reveals a non-linear distortion in intensity. Investigation of the measured CVi /C12 ratios comparing with theoretical ones shows that the non-linearity can be attributed to low intensity signal suppression of low abundance peptide peaks. We find that the suppression is homogenous for different isotopes of identical peptides but non-homogenous for different peptides. We developed an iterative correction algorithm that corrects the intensity distortions for peptides with relatively high abundance. This algorithm can be applied in a wide range of applications using FTMS.\",\"PeriodicalId\":275098,\"journal\":{\"name\":\"2010 IEEE International Conference on Bioinformatics and Biomedicine (BIBM)\",\"volume\":\"82 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2010 IEEE International Conference on Bioinformatics and Biomedicine (BIBM)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/BIBM.2010.5706567\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2010 IEEE International Conference on Bioinformatics and Biomedicine (BIBM)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/BIBM.2010.5706567","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Iterative correction of suppressed peptide profiles from FTMS measurements
Analysis of peptide profiles from a Liquid Chromatography Fourier Transform Mass Spectrometry (LC/FTMS) measurement reveals a non-linear distortion in intensity. Investigation of the measured CVi /C12 ratios comparing with theoretical ones shows that the non-linearity can be attributed to low intensity signal suppression of low abundance peptide peaks. We find that the suppression is homogenous for different isotopes of identical peptides but non-homogenous for different peptides. We developed an iterative correction algorithm that corrects the intensity distortions for peptides with relatively high abundance. This algorithm can be applied in a wide range of applications using FTMS.
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