用藏红花红和靛蓝-微胭脂红对植物细胞周期进行鉴别染色。

D Swain, D N De
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引用次数: 2

摘要

红花-靛蓝-微胭脂碱(SIPC)三色染色技术可用于区分洋葱根尖细胞周期的不同阶段。将组织先用福尔马林再用苦味酸固定,SIPC染色,可见间期细胞核明显分化为绿色、橙色、红色和棕色四种颜色。用水晶紫代替红花红可以产生类似的间期细胞核分化为绿色、浅蓝色、蓝色和深蓝色的模式。使用3h -胸腺嘧啶作为DNA前体的放射自显影研究证明了SIPC染色技术的可靠性。所有橙色和红色的细胞核都被标记,因此处于细胞周期的S期。几乎所有的绿色细胞核都没有标记,可能属于G1。大部分未标记的较大的棕色核可以认为处于G2期。
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Differential staining of the cell cycle of plant cells using safranin and indigo-picrocarmine.

A trichrome staining technique using safranin-indigo-picrocarmine (SIPC) can be used to distinguish the various stages of the cell cycle in onion root tip. When the tissue was fixed first in formalin followed by picric acid and stained in SIPC, a clear differentiation of interphase nuclei into four color classes, viz., green, orange, red and brown can be recorded. Replacing crystal violet for safranin produces a similar pattern of differentiation of interphase nuclei into green, light blue, blue and deep blue. Autoradiographic study using 3H-thymidine as a DNA precursor demonstrates the reliability of the SIPC staining technique. All the orange and red nuclei are found to be labelled and therefore are in S phase of the cell cycle. Almost all the green nuclei are unlabelled and may be assigned to G1. The larger brown nuclei which are mostly unlabelled can be considered in G2 phase.

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