一种去除环氧树脂的溶液。

T Iwadare, E Harada, S Yoshino, T Arai
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引用次数: 23

摘要

介绍了一种用于低碱浓度的树脂溶解溶液的研制。冠醚溶解在二甲亚砜中产生超碱性醇氧阴离子。5分钟的治疗导致Epon 812包埋的肾活检标本的树脂完全去除。标本经勒夫勒亚甲基蓝染色。周期酸-甲基苯丙胺银染色和吉姆萨染色结果良好。应用PAS反应和随后的苏木精反染色对诊断是可行的。
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A solution for removal of resin from epoxy sections.

Development of a resin-dissolving solution for use at low alkali concentrations is described. Crown ether dissolved in dimethyl-sulfoxide produces a superbasic alkoxide anion. A five minute treatment resulted in complete resin removal from kidney biopsy specimens embedded in Epon 812. Specimens were well stained by Loeffler's methylene blue. Periodic acid-methenamine silver and Giemsa stains yielded good results. Application of PAS reaction and subsequent hematoxylin counterstaining was practicable for diagnosis.

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Effect of sodium hexanitrocobaltate (III) decomposition on its staining of intracellular potassium ions. A modified mallory-cason staining procedure for large cryosections. A simple procedure to visualize osmicated storage lipids in semithin epoxy sections of plant tissues. Standard specimens for stain calibration: application to Romanowsky-Giemsa staining. Localization of plant lipids for light microscopy using p-phenylenediamine in tissues of Arachis hypogaea L.
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