臭草和牛粪混合提取物对尖孢镰刀菌病原菌的抑制作用

A. Akintokun, P. Akintokun, A. Oloyede
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摘要

研究了牛粪堆肥提取物和臭虫对尖孢镰刀菌的防治效果。本研究以暹罗草(Chromolaena odorata)和牛粪为原料制备了3种堆肥样品。样品A由牛粪和暹罗杂草按100g: 100g的比例配制而成,样品B由切碎的暹罗杂草200g配制而成,样品C含有200g牛粪。这三个样品在塑料桶中进行堆肥,每个样品重复三次。在1、10、30和60天内,对桶内的内容物进行定期翻转和监测。采用标准程序对堆肥进行微生物学分析。采用琼脂孔扩散法测定了灭菌后的堆肥提取物对黄芽孢杆菌的抑菌活性。细菌、大肠菌群和真菌负荷分别为1.50 - 9.0 Ã - 107 cfu/ml、0.3 - 6.0Ã - 107 cfu/ml和0.1 - 2.50 Ã - 107 cfu/ml。堆肥提取物在不同堆肥天数对尖孢镰刀菌的抑制活性随堆肥天数的增加而增加。在第60天,以臭草为原料制备的堆肥提取物的抑制区最高,在第60天,以牛粪与臭草混合制备的堆肥提取物的抑制区次之。24h堆肥提取液均无抗真菌活性。提取24 h和对照组的疾病严重程度最高。在发酵60 d后,所有提取物都是健康的。因此,研究显示了臭草和牛粪提取物对许多作物的尖孢镰刀菌的抑制潜力。
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INHIBITION OF Fusarium oxysporum Pathogenic Fungus USING COMPOST EXTRACT FROM Chromolaena odorata (SIAM WEED) AND COW DUNG
The study was conducted to evaluate the efficacy of composts extract from cow dung and Chromolae-na odorata in controlling Fusarium oxysporum. Three compost samples were prepared in this study from Siam weed (Chromolaena odorata) and cow dung. Sample A was prepared from Cow dung and siam weed at ratio 100g: 100g, Sample B was prepared from 200g chopped siam weed and sample C contained 200g cow dung. These three samples were composted in plastic drums perforated for aera-tion and each sample were replicated three times. The content in the drums were regularly turned and monitored for 1, 10, 30 and 60 days. The microbiological analysis of the composts were determined using standard procedures. The inhibitory activities of the sterilised compost extracts on the F. ox-ysporum were determined using agar well diffusion method. The bacterial, coliform and fungal loads ranged from 1.50 – 9.0 × 107 cfu/ml, 0.3 – 6.0× 107 cfu/ml and 0.1 – 2.50 × 107 cfu/ml respectively. Inhibitory activities of the compost extracts on F. oxysporum at different days of composting increased with days of composting. The highest zone of inhibition was recorded by extract from compost pre-pared from C. odorata at 60 days of composting, closely followed by extract from compost prepared from mixture of cow dung and C. odorata at 60 days of composting. No antifungal activity was found in all extracts from the 24h-composts. Highest disease severity was recorded in extract of 24 h and on control. All extract at 60 days of fermentation were healthy The study therefore revealed the potentials of extracts from Chromolaena odorata and cow dung for the inhibition of Fusarium oxysporum of many crops.
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