尼日尔州明纳当地生产的虎坚果饮料(Kunuaya)中多重耐药细菌的研究

U. Oyedum, J. Agbala
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摘要

多药耐药(MDR)细菌在民众、食用食品和饮料中的优势地位正迅速成为大多数社区关注的主要问题。三(3)本地配制的饮料从三个地点无菌收集和运输到联邦理工大学微生物实验室,米纳。将样品依次稀释,通过涂布板法接种于各种培养基上。根据革兰氏反应和其他生化试验鉴定分离的细菌。采用Muller hinton琼脂圆盘扩散法对分离的细菌进行药敏试验。结果显示,在所有当地制备的饮料中,来自联邦理工大学Minna, Bosso校区的虎坚果饮料(Kunuaya) (3.9 x103)的微生物数量最高。分离鉴定出多种病原菌,其中以大肠杆菌、金黄色葡萄球菌和沙门氏菌的发生率最高(25%)。抗生素敏感性试验显示,所有分离的细菌都具有多重耐药性,因此对一般公众的健康,特别是这些当地配制的饮料的经常消费者的健康构成重大威胁。因此,尼日利亚的食品监管机构需要进行充分和持续的监测,以遏制与耐多药细菌有关的传播和感染。
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The study of multidrug resistant bacteria from locally produced tiger nut drinks (Kunuaya) sold in Minna, Niger State
The predominance of multidrug resistant (MDR) bacteria among the populace, edible foods and drinks is fast becoming the major concern in most communities. Three (3) locally prepared drinks from three locations were aseptically collected and transported to the Microbiology Laboratory of Federal University of Technology, Minna. Samples were serially diluted and were inoculated on various media through the spread plate method. The bacterial isolates were identified based on their Gram reaction and other biochemical tests. The antibiotic susceptibility tests were carried out for the bacterial isolates using the disc diffusion method on Muller hinton agar. The result revealed that out of all the locally prepared drinks sampled tiger-nut drink (Kunuaya) (3.9 x103) from Federal University of Technology, Minna, Bosso campus had the highest microbial count. Various bacterial pathogens were isolated and identified with Escherichia coli, Staphylococcus aureus and Salmonella sp having the highest frequency of occurrence (25%).The antibiotic susceptibility tests revealed that all bacterial isolates were Multidrug resistant and as such are a great threat to the health of the general public especially the regular consumers of these locally prepared drinks. Hence, there is a need for adequate and continuous surveillance by food regulatory bodies in Nigeria, to curtail the spread and infections associated with Multidrug resistant bacteria.
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