Chertov OYu, I N Telezhinskaya, E V Zaitseva, T B Golubeva, V V Zinov'ev, L G Ovechkina, L B Mazkova, E G Malygin
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引用次数: 0
摘要
用非离子清洁剂Nonidet P-40从病毒粒子中提取牛痘病毒主要免疫优势包膜蛋白p35。用溴化氰裂解蛋白质。分离得到4个均质肽,并测定其n端氨基酸序列。计算机检索蛋白质序列数据库发现,所确定的序列与牛痘病毒基因组HindIII-H片段ORF H3的44-63、144-149、154-165和224-238序列完全一致(Rosel et al 1986)。因此,已经确定牛痘病毒的免疫优势蛋白p35是由牛痘病毒基因组HindIII-H片段中的基因编码的。
Amino acid sequence determination of vaccinia virus immunodominant protein p35 and identification of the gene.
A major immunodominant envelope protein of vaccinia virus (protein p35) was purified by extraction from virions with the nonionic detergent Nonidet P-40. The protein was cleaved with cyanogen bromide. Four homogeneous peptides were isolated and their N-terminal amino acid sequences determined. A computer search of a protein-sequence data bank revealed complete identity of the determined sequences with sequences 44-63, 144-149, 154-165, and 224-238 of ORF H3 of the HindIII-H fragment of the vaccinia virus genome (Rosel et al 1986). It has therefore been established that the immunodominant protein p35 of vaccinia virus is encoded by the gene in the HindIII-H fragment of the vaccinia virus genome.