罂粟碱对犬颈外静脉内皮细胞收获的影响。

Blood vessels Pub Date : 1991-01-01 DOI:10.1159/000158895
T J Ranval, L E Townsend, R Fietsam, P J Bendick, D M Reitz-Vick, J L Glover
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引用次数: 3

摘要

内皮细胞播种程序已经发展到用于外周血管疾病的假体旁路移植;然而,由于目前效率低下的细胞收获技术,需要高比例的静脉与移植物面积。本研究的目的是确定使用罂粟碱(一种平滑肌细胞松弛剂)是否会影响犬颈外静脉内皮细胞的数量或活力。在组织培养基中加入0.12 mg/ml的罂粟碱溶液,在解剖和切除过程中浸泡静脉,活细胞产量为2.20 +/- 1.16(细胞× 10(4)/cm2)。对照组静脉采用标准解剖技术,产率为0.97 +/- 0.40 (p = 0.025)。单独浸泡在组织培养基中解剖的第二组静脉产量为1.82 +/- 0.75,而罂粟碱收获的静脉产量为2.73 +/- 0.45 (p = 0.009)。各组的细胞存活率没有显著差异:罂粟碱组、对照组和培养基组的细胞存活率分别为73%、70%和76%。罂粟碱收获的细胞和单独培养基收获的细胞在组织培养中分别在9.8 +/- 1.1和9.9 +/- 0.9天达到95%的融合。与传统手术技术相比,罂粟碱的使用使切除静脉段的内皮细胞产量增加了一倍以上,而不会对细胞培养的活力或生长速度产生不利影响。
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Effect of papaverine on endothelial cell harvest from canine external jugular veins.

Endothelial cell seeding procedures have been developed to line prosthetic bypass grafts used in peripheral vascular disease; however, because of current inefficient cell harvest techniques a high ratio of vein-to-graft area is necessary. This study was done to determine if the use of papaverine, a smooth muscle cell relaxant, would affect the number or viability of endothelial cells harvested from canine external jugular veins. Using a 0.12 mg/ml solution of papaverine in tissue culture medium to bathe the veins during dissection and excision, the viable cell yield was 2.20 +/- 1.16 (cells x 10(4)/cm2). A control group of veins using standard dissection technique gave a yield of 0.97 +/- 0.40 (p = 0.025). A second group of veins dissected while bathed in tissue culture medium alone gave a yield of 1.82 +/- 0.75, compared to a yield of 2.73 +/- 0.45 for papaverine harvested veins (p = 0.009). Percent cell viability was not significantly different for any of the groups: 73, 70, and 76% for papaverine, control and media only veins, respectively. The papaverine-harvested cells and those harvested with medium alone grew to 95% confluence in tissue culture in 9.8 +/- 1.1 and 9.9 +/- 0.9 days, respectively. Compared to conventional surgical techniques, use of papaverine more than doubled the endothelial cell yield from excised vein segments without adversely affecting viability or rate of growth in cell culture.

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