M Watanabe, K Izumiya, T Sato, K Yoshino, N Nakagawa, M Ohoishi, M Hoshino
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引用次数: 0
摘要
摘要对1987-1990年北中研究所生产的14批无细胞百日咳-白喉-破伤风(AC-PDT)疫苗的质量进行了调查。HSU、LPU和BWDU的几何平均值分别为0.078、0.257和7.33 / ml。效价高于14 IU / ml，说明北中AC-PDT疫苗的一致性。采用ELISA法测定了一、二次接种AC-PDT疫苗小鼠的抗体应答。一次注射后，所有免疫小鼠对FHA和PT均有IgG抗体应答(P < 0.001)。与此相反，免疫增强剂注射后，免疫球蛋白g抗体对菌膜2的反应滴度显著升高(P < 0.001)。结果表明，北中AC-P疫苗以蛋白、PT和FHA为主要抗原，少量凝集素为次要抗原。
Comparative biological activities of acellular pertussis vaccines produced by Kitasato.
The quality of 14 lots of acellular pertussis-diphtheria-tetanus (AC-PDT) vaccines manufactured by the Kitasato Institute during the period 1987-1990 were investigated. The geometric means of HSU, LPU, and BWDU were 0.078, 0.257, and 7.33 per ml respectively. The potency was higher than 14 IU per ml. These results indicated the consistency of the Kitasato AC-PDT vaccines. The antibody response to the AC-PDT vaccines was measured in primary and secondary vaccinated mice by ELISA. IgG antibody response to FHA and PT was obtained in all immunized mice (P less than 0.001) after the primary injection. In contrast, IgG antibody response to fimbriae 2 showed a significant titer rise (P less than 0.001) after the booster injection. The results indicated that the Kitasato AC-P vaccines consisted of protein, PT and FHA as the major antigens, and a little agglutinogen as the minor antigen.
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