G Schäffner, E Seeger, H D Volk, R Fischer, S Kiowski
{"title":"[一种测定毛细血管血中淋巴细胞亚群的全血显微法，与其他间接免疫荧光法的比较]。","authors":"G Schäffner, E Seeger, H D Volk, R Fischer, S Kiowski","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Six lymphocyte antigens were detected with monoclonal antibodies in 0.2 ml capillary blood by a whole blood micromethod. After the lysis of erythrocytes of one drop of blood the remaining cells were layered on slides and were stained by indirect immunofluorescence. The relative and absolute counts of the lymphocyte subsets were of normal value and did not differ from the counts of flow cytometry and of slide and tube tests.</p>","PeriodicalId":7505,"journal":{"name":"Allergie und Immunologie","volume":"37 2","pages":"103-8"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[A whole-blood micromethod to determine lymphocyte subsets in capillary blood, comparison with other methods of indirect immunofluorescence].\",\"authors\":\"G Schäffner, E Seeger, H D Volk, R Fischer, S Kiowski\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Six lymphocyte antigens were detected with monoclonal antibodies in 0.2 ml capillary blood by a whole blood micromethod. After the lysis of erythrocytes of one drop of blood the remaining cells were layered on slides and were stained by indirect immunofluorescence. The relative and absolute counts of the lymphocyte subsets were of normal value and did not differ from the counts of flow cytometry and of slide and tube tests.</p>\",\"PeriodicalId\":7505,\"journal\":{\"name\":\"Allergie und Immunologie\",\"volume\":\"37 2\",\"pages\":\"103-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Allergie und Immunologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Allergie und Immunologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[A whole-blood micromethod to determine lymphocyte subsets in capillary blood, comparison with other methods of indirect immunofluorescence].
Six lymphocyte antigens were detected with monoclonal antibodies in 0.2 ml capillary blood by a whole blood micromethod. After the lysis of erythrocytes of one drop of blood the remaining cells were layered on slides and were stained by indirect immunofluorescence. The relative and absolute counts of the lymphocyte subsets were of normal value and did not differ from the counts of flow cytometry and of slide and tube tests.
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