Ultrasonic baths as substitutes for shaking incubator baths.

An easily assembled incubation bath for enzyme work is described. The bath is made from commercially available units: an ultrasonic bath of the type used for cleaning and a temperature-controlling device. The device is not only much cheaper, quieter, and more compact than a commercially built shaking-type bath, but is also gives superior mixing of heterogeneous enzyme incubation samples, particularly those containing tissue homogenates or subcellular particles.