[牙髓细胞的体外培养]。

J Müller, G Bruckner, W Hörz
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引用次数: 0

摘要

我们研究的目的是培养牙髓的细胞成分。在离体培养牙髓组织过程中观察到成纤维细胞样细胞的迁移和增殖。然而,培养基的营养变化不能诱导成牙细胞的有丝分裂活性。成纤维细胞样细胞的增殖在原代培养和第一次传代培养中发生变化。相比之下,在第二次亚文化中,有一个同质的生长过程。因此,第二代培养的生长速度适合于牙科材料的生物学评价。
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[In vitro culture of pulp cells].

The purpose of our study was the culture of cellular elements of the pulp. Migration and proliferation of fibroblast-like cells can be observed during the in vitro culture of pulpal tissue. However, no mitotic acticity of the odontoblasts could be induced by nutritive variations of the culture medium. The proliferation of the fibroblastlike cells varied during the primary culture and the first subculture. By contrast, during the second subculture there is a homogeneous process of growth. The growth rate of the second subcultures is therefore suitable for a biological evaluation of dental materials.

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