非侵入性野生动物和栖息地健康评估和保护的现场和实验室分析

C. Knott, Amy M. Scott, Caitlin A O'Connell, T. Susanto, Erin E. Kane
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引用次数: 0

摘要

实验室方法越来越多地被用于偏远的野外营地,或在流动野外调查期间,以帮助野生动物保护。本章解释了现场实验室如何允许样品制备和保存的技术进步,以及低技术和高技术的现场分析。它强调了如何将实地样本用于健康和生理分析,包括测量植物性食物的营养成分,评估尿液中的激素、c肽和酮类,检查粪便寄生虫,以及使用遗传技术确定个体身份、亲缘关系和种群遗传多样性。我们解释了生理和健康的测量如何有望大大提高我们对野生动物疾病流行与人为干扰之间关系的理解。这组作者对印度尼西亚极度濒危野生猩猩的研究提供了一个说白了的案例研究,利用实地实验室快速分析猩猩的健康状况,例如评估尿液中的能量平衡指标和粪便中的寄生虫流行情况。此外,本章还展示了如何利用实验室方法和设备的技术进步来确定食品的营养成分、测量类固醇激素和c肽并揭示遗传关系,从而从实地收集的样品中获得新的信息。了解这些变量如何影响野生动物的健康和生存能力,是物种保护的重要工具,有助于使长期研究具有可持续性,提供能力建设机会,并与当地合作伙伴建立关系。在不久的将来,持续的技术进步应该会给现场实验室带来更多的能力,提供更容易获得和更容易获得的数据来帮助保护。
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Field and laboratory analysis for non-invasive wildlife and habitat health assessment and conservation
Laboratory methods are increasingly being used in remote field camps, or during mobile field surveys, to aid in wildlife conservation. This chapter explains how field laboratories have allowed for technological advances in sample preparation and preservation, and for both low and high-tech on-site analysis. It highlights how field samples can be used for health and physiological analyses, including measuring the nutritional content of plant foods, assessing hormones, C-peptide, and ketones from urine, examining faecal parasites, and using genetic techniques to determine individual identity, relatedness, and population genetic diversity. We explain how measurements of physiology and health promise to greatly improve our understanding of the relationship between disease prevalence in wild animals and anthropogenic disturbances. The authors’ research with critically endangered wild orangutans in Indonesia provides an illustrative case study, using field laboratories for rapid analysis of orangutan health status, such as assessing indicators of energy balance from urine and parasite prevalence from faeces. In addition, the chapter shows how new information can be gained from field-collected samples by taking advantage of technological advances in laboratory methodology and equipment to determine the nutrient content of foods, measure steroid hormones, and C-peptide and reveal genetic relationships. Understanding how these variables impact wildlife health and viability provides a critical tool for species conservation and helps make long-term research sustainable, provides capacity building opportunities, and forges relationships with local partners. Continued technological advances in the near future should bring more capability into field laboratories, providing data to aid conservation that is easier to obtain and more accessible.
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