超声波法和索氏法提取香根草油的性能研究

H. Haryono, E. Ernawati, Adella Hayu Erliana
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摘要

本研究旨在采用超声提取和索氏提取两种方法提取香根草油。根据提取工艺条件对香根草油得率和质量参数的影响,比较了两种提取方法的提取性能。测定了香根草油的质量,并与SNI 06- 2386-2006进行了比较。指定的质量参数是颜色、气味、密度、折光率和在95%乙醇中的溶解度。采用气相色谱-质谱联用方法对香根草油中化合物的组成进行了鉴定。结果表明,超声提取比索氏提取更有效。超声提取和索氏提取的出油率分别为3.98±0.58%和3.95±0.81%。在超声辅助提取中进行的提取时间比索氏提取缩短约3/10倍,溶剂用量减少约2/3。质量方面,超声提取香根草油根据试验参数符合SNI 06-2386-2006质量标准。气相色谱-质谱分析表明,超声提取香根草精油的主要成分为苦参酸(36.40%)、齐酮(13.89%)、诺卡酮(1.92%)、γ-亚麻烯(1.57%)、α-香根草酮(1.43%)、异长叶烯(1.32%)、δ-癸二烯(0.86%)和香根草烯(0.64%)。
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Performance of Vetiver Oil Extraction by Ultrasonication and Soxhletation Methods
This study aims to extract vetiver oil by alternative extraction methods, namely ultrasonic extraction and soxhlet extraction. The performance of both extraction methods is compared based on the effect of extraction operation conditions with yield and quality parameters of vetiver oil produced. The quality of vetiver oil was determined and compared with SNI 06- 2386-2006. The specified quality parameters are color, odor, density, refractive index, and solubility in 95% ethanol. The composition of compounds in vetiver oil was identified by GC-MS method (Gas Chromatography-Mass Spectrometry). The results showed that ultrasonic extraction performed more effectively than soxhlet extraction. The ultrasonic extraction and soxhlet extraction gave an oil yield of 3.98±0.58% and 3.95±0.81%. In the extraction with ultrasonic aid performed in extraction time about 3/10 times shorter and solvent consumption about 2/3 times less compared with soxhlet extraction. In terms of quality, vetiver oil from ultrasonic extraction has eligible with the quality standard according to SNI 06-2386-2006 based on the test parameter. The GC-MS showed that vetiver oil from ultrasonic extraction composed of the mayor compounds of khusenic acid (36.40%), zierone (13.89%), nootkatone (1.92%), γ-selinene (1.57%), α-vetivone (1.43%), isolongifolene (1.32%), δ-cadinene (0.86%), and khusimene (0.64%).
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