Effect of acclimatization rate on biogas production from anaerobic digestion of biodiesel waste products

Anaerobic digestion can be used to sustainably treat the organic byproducts of the biodiesel process (crude glycerol and biodiesel wastewater) while generating a renewable natural gas to be used for heating or electricity generation. The purpose of this thesis was to (1) investigate the possibility of co-digestion of biodiesel byproducts without use of external substrates or pretreatment and (2) assess the impact of various acclimatization rates on the stability and efficiency of such a system. Two inocula (effluent from a wastewater treatment plant digester and from an agro-industrial waste digester) and two acclimatization rates were studied. The results showed that co-digestion of crude glycerol and biodiesel wastewater at high organic loading rates(up to 6.8 g COD L-1day-1)is possible without addition of other substrates or pretreatment.Thecumulative biogas production of the digesters using inoculum from the agro-industrial waste digester was statistically greater than the digesters using the wastewater treatment plant digester, indicating that similar inoculum could be useful for additional experiments.In addition,maximum efficiency due to a slower rate of acclimatization was higher for both inocula, up to a maximum average daily biogas yield of 621 mL biogas g-1COD added.Finally, comparison of two methods for measuring gas production (mass difference and volumetrically using a syringe) revealed a reasonable correlation(R2= 0.97)between the methods. Additional validation could lead to use of the mass difference method as a validation method or an alternative gas production measurement method.