Frap法分析罗望子叶乙酸乙酯提取物的植物化学及抗氧化活性

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摘要

氧化应激的特点是自由基的产生和随后被内源性抗氧化剂消除的不平衡。它是几种疾病的特征,特别是在进展阶段,可导致致命的影响。本研究旨在研究柽柳的植物化学成分和抗氧化能力,并评估其作为抗氧化应激相关疾病的候选植物的能力。重量法检测和定量植物化学成分,还原力法测定抗氧化潜能。皂苷、甾体和黄酮类化合物的含量分别为6.83±0.44%、4.30±0.60%和10.17%±0.60%,不含生物碱、苷类和萜类。抗氧化试验表明,提取物和标准品(抗坏血酸)的吸光度都呈浓度依赖性增加。抗坏血酸具有较高的吸光度。100%浓度下,样品吸光度为0.388±0.022,低于40%浓度下抗坏血酸的吸光度0.411±0.009。由此可见,罗望子叶可用于控制与氧化应激相关的疾病,其抗氧化潜力归因于叶中的植物化学成分。然而,需要进一步的研究来确定确切的化合物及其作用方式。
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Phytochemical Analysis and Antioxidant Potential of Ethylacetate Extract of Tamarindus Indica (Tamarind) Leaves by Frap Assay
Oxidative stress is characterized by an imbalance in the generation of free radicals and their subsequent elimination by endogenous antioxidants. It is a characteristic of several diseases, especially during the progression stage, which can lead to fatal effects. This study aims to investigate the phytochemical components and antioxidant capability of Tamarindus indica and assess its capability as a candidate for managing diseases associated with oxidative stress. The gravimetric method detected and quantified phytochemicals, while the reducing power assay determined the antioxidant potential. Saponins, steroids, and flavonoids were detected in 6.83 ±0.44, 4.30 ±0.60, and 10.17% ±0.60, respectively, without alkaloids, glycosides, and terpenoids. The antioxidant test showed a concentration-dependent increase in absorbance of both the extract and standard (Ascorbic acid). However, Ascorbic acid had higher absorbance. At 100% concentration, the sample had an absorbance of 0.388 ±0.022, which was lower than the absorbance of Ascorbic acid (0.411 ±0.009) at 40% concentration. It can be concluded that Tamarind leaves could be utilized to manage diseases associated with oxidative stress, evidenced by their antioxidant potential credited to the phytochemical content of the leaves. However, there is a need for further studies to ascertain the exact compounds and their modes of action.
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