Flavonoids in psidium guajava L. leaves

Introduction: The leaves have been extensively investigated in the biology activity in human and animal health, where important metabolites such as phenols have been associated, of which the following stand out: flavonoids, tannins among other compounds, however this document aims to locate the anatomical location and analyze the content of major flavonoids in the mature and immature guava leaf. The study was complemented with the analysis of the ethyl ether and butanol extracts; The two types of leaves with their respective extracts were analyzed by thin layer chromatography, which allowed them to be detected and their concentration estimated by liquid chromatography. The study indicated that the mature leaf shows a large amount of flavonoids located in the adaxial and abaxial epidermis, as well as in glands, while in the immature leaf in the adaxial epidermis and in the abaxial epidermis it only presented a minimal proportion of them. Conclusion: Total flavonoid concentrations range from 2610 mg *kg-1 in the mature leaf to 2016 mg* kg-1 for the immature leaf, quercetin being the one that was found and observed in the highest proportion in both leaves.