Meliaceae植物的代谢化合物与水肿毒性发作水平的关系

J. Sembiring
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引用次数: 0

摘要

本研究旨在确定meliaceae植物中代谢物化合物与Hypsipyla sp偏好的关系。本研究在Sam Ratulangi州立大学数学与自然科学学院化学实验室,Manado BALITKA实验室,Gorontalo州立大学数学与自然科学学院化学实验室进行,并将样品送到日本仙台药物实验室查看核磁共振结构。本研究采用拉丁方设计,5个处理,5个重复。各处理植株总数均为250株。二级代谢物的测定分5个阶段进行,即:1)样品采集和处理,2)提取,3)纯化,4)植物化学试验,5)紫外-可见和红外分光光度计鉴定化合物。所使用的样品材料为干样,包括大红木和小红木,分别为langsat、duku、mindi和红木叶子。对lanum、duku、chinaberry、大红木和小红木样品的正己烷、乙酸乙酯和水馏分的植物化学筛选结果显示,黄酮类化合物和类固醇呈阳性,生物碱、萜类化合物和皂苷呈阴性。分析结果表明,小红木的淀粉含量高达43.72 g/mL,小红木的脂肪含量高达24.15%,Langsat的蛋白质含量高达9.01 g/mL。相较于其他植物,暗夜蛾对小型红木的偏好程度更高。与其他植物相比,红木气孔的数量调节了蒸发的速度,蒸发也会释放次生代谢化合物作为引诱剂,吸引昆虫到植物上。红木中主要的类黄酮基团为槲皮素3-O-β- d -半乳糖苷、槲皮素3-O-β- d -葡萄糖苷和槲皮素3-O-α- l -鼠李糖苷。关键词:植物化学物质;植物代谢产物;槲皮素;桃花心木;Hypsipyla sp。
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Hubungan Senyawa Metabolit Pada Tanaman Meliaceae Terhadap Tingkat Serangan Hypsipyla robusta
This study aims to determine the relationship of metabolite compounds in meliaceae plants to the preference of Hypsipyla sp. This research was conducted at the Chemistry Laboratory, Faculty of Mathematics and Natural Sciences, Sam Ratulangi State University, Manado BALITKA Laboratory, Chemistry Laboratory, Faculty of Mathematics and Natural Sciences, State University of Gorontalo and sending samples to the Pharmacy Laboratory of Sendai Japan to see the NMR structure. This study was designed in a Latin square design with 5 treatments and 5 replications. The total number of plants in all treatments was 250 plants. The determination of secondary metabolites was carried out in five stages, namely: 1) sample collection and processing, 2) extraction, 3) purification, 4) phytochemical test, 5) identification of compounds using UV-Vis and IR spectrophotometers. The sample materials used were dry samples of langsat, duku, mindi, and mahogany leaves consisting of large mahogany and small mahogany. The results of phytochemical screening on samples of lansium, duku, chinaberry, large mahogany and small mahogany in the n-hexane, ethyl acetate and water fractions showed positive results for flavonoids and steroids, while for alkaloids, terpenoids and saponins showed negative results. The results of the analysis showed that small mahogany plants contained high starch content of 43.72 g/mL, high fat content of small mahogany plants of 24.15% and high protein content of Langsat plants of 9.01 g/mL. The level of preference Hypsipyla sp prefers small mahogany plants compared to other plants. The number of mahogany stomata compared to other plants regulates the rate of evaporation in which will also release secondary metabolic compounds as attractants to attract insects to plants. The main flavonoid groups in mahogany are Quercetin 3-O-β-D-galactopyranoside, Quercetin 3-O-β-D-glucopyranoside, and quercetin 3-O-α-L-rhamnopyranoside.  Key words: phytochemicals; plant metabolites; quercetin; mahogany; Hypsipyla sp.
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