E Köhler, G Varnai, S Knospe, W Förster, D Michaelis, H Wanka
{"title":"血小板活化因子拮抗剂bn52021对1型(胰岛素依赖)糖尿病患者单核细胞和血清抗胰岛细胞毒性的影响","authors":"E Köhler, G Varnai, S Knospe, W Förster, D Michaelis, H Wanka","doi":"10.1159/000235472","DOIUrl":null,"url":null,"abstract":"<p><p>The effect of platelet-activating factor (PAF) antagonist BN 52021 (0.06-2.5 mM) on the cytotoxic activity of mononuclear cells (MNC) from newly diagnosed type 1 diabetic patients against 51Cr-labeled Langerhans islets from neonatal rats was investigated in a 6-hour cytotoxicity test. A dose-dependent inhibition of anti-islet cytotoxicity by BN 52021 was observed. The suppression of the islet lysis was significant at a concentration of 0.6 mM BN 52021. During a 4-day cell culture, BN 52021 had no inhibitory effect on the antigen-mediated triggering of immunocytes with anti-islet cytotoxicity. The results suggest that the drug is only effective during immunocytolytic reactions of MNC against pancreatic islets. A PAF-independent action of BN 52021 can not be excluded at present.</p>","PeriodicalId":13810,"journal":{"name":"International archives of allergy and applied immunology","volume":"95 4","pages":"352-5"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000235472","citationCount":"1","resultStr":"{\"title\":\"Effects of the platelet-activating factor antagonist BN 52021 on anti-islet cytotoxicity of mononuclear cells and serum from type 1 (insulin-dependent) diabetic patients.\",\"authors\":\"E Köhler, G Varnai, S Knospe, W Förster, D Michaelis, H Wanka\",\"doi\":\"10.1159/000235472\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The effect of platelet-activating factor (PAF) antagonist BN 52021 (0.06-2.5 mM) on the cytotoxic activity of mononuclear cells (MNC) from newly diagnosed type 1 diabetic patients against 51Cr-labeled Langerhans islets from neonatal rats was investigated in a 6-hour cytotoxicity test. A dose-dependent inhibition of anti-islet cytotoxicity by BN 52021 was observed. The suppression of the islet lysis was significant at a concentration of 0.6 mM BN 52021. During a 4-day cell culture, BN 52021 had no inhibitory effect on the antigen-mediated triggering of immunocytes with anti-islet cytotoxicity. The results suggest that the drug is only effective during immunocytolytic reactions of MNC against pancreatic islets. A PAF-independent action of BN 52021 can not be excluded at present.</p>\",\"PeriodicalId\":13810,\"journal\":{\"name\":\"International archives of allergy and applied immunology\",\"volume\":\"95 4\",\"pages\":\"352-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1159/000235472\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International archives of allergy and applied immunology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1159/000235472\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International archives of allergy and applied immunology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000235472","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
通过6小时的细胞毒试验，研究了血小板活化因子(PAF)拮抗剂BN 52021 (0.06-2.5 mM)对新诊断1型糖尿病患者单核细胞(MNC)对新生大鼠51cr标记朗格汉斯胰岛的细胞毒活性的影响。观察到BN 52021对抗胰岛细胞毒性的剂量依赖性抑制作用。在浓度为0.6 mM BN 52021时，对胰岛溶解的抑制是显著的。在4天的细胞培养过程中，BN 52021对抗原介导的具有抗胰岛细胞毒性的免疫细胞的触发没有抑制作用。结果表明，该药仅在MNC对胰岛的免疫细胞溶解反应中有效。目前不能排除BN 52021独立于paf的行动。
Effects of the platelet-activating factor antagonist BN 52021 on anti-islet cytotoxicity of mononuclear cells and serum from type 1 (insulin-dependent) diabetic patients.
The effect of platelet-activating factor (PAF) antagonist BN 52021 (0.06-2.5 mM) on the cytotoxic activity of mononuclear cells (MNC) from newly diagnosed type 1 diabetic patients against 51Cr-labeled Langerhans islets from neonatal rats was investigated in a 6-hour cytotoxicity test. A dose-dependent inhibition of anti-islet cytotoxicity by BN 52021 was observed. The suppression of the islet lysis was significant at a concentration of 0.6 mM BN 52021. During a 4-day cell culture, BN 52021 had no inhibitory effect on the antigen-mediated triggering of immunocytes with anti-islet cytotoxicity. The results suggest that the drug is only effective during immunocytolytic reactions of MNC against pancreatic islets. A PAF-independent action of BN 52021 can not be excluded at present.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
