用一般植物生长参数对马铃薯品种进行离体评价的方法

P. S. Michael, Davinah Lepatu
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引用次数: 2

摘要

本文介绍了从国际马铃薯中心(IPC)引进的17个马铃薯品种和巴布亚新几内亚当地品种红杉(Sequoia)的分生组织尖端(0.02 mm)的微繁殖过程,并基于一般植物生长参数(PGP);芽、根、叶、节和高度)。利用这一过程,该研究能够将被测试的品种分为三个可能不同的遗传组。LSD平均分离度在评价参数间也有极显著差异(p < 0.05);显示这些品种之间存在的差异遗传变异。回归分析进一步表明,茎部对植株生长的总体格局有较强的影响,这与茎部在外植体上最先增殖的PGP的观察结果相吻合。关键词:马铃薯品种,微繁,植株生长参数,评价,离体
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A procedure for in vitro evaluation of potato varieties using general plant growth parameters
Presented in this paper is a procedure in which meristem tips (0.02 mm) of seventeen recently introduced potato varieties from the International Potato Center (IPC) and a local variety (Sequoia) from PNG were micropropagated and evaluated in vitro based on general Plant Growth Parameters (PGP; shoots, roots, leaves, nodes and height). Using this procedure, the study was able to place the tested varieties into three potentially diverse genetic groups. LSD mean separations also showed highly significant differences (p < 0.05) among the evaluated parameters; showing the differential genetic variations that exist among these varieties. Regression analysis further indicated that shoot has strong influence on general pattern of plant growth, and coincides with observations that shoot was the first PGP to proliferate on the explants cultured. Keywords: Potato varieties, Mircopropagation, Plant growth parameters, Evaluation, In vitro
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