纳米碳对细菌的影响及其在海水污染检测中的应用前景纳米碳作为抗生素和海水生物传感器

Akerke Altaikyzy, Haiyan Fan, Yingqiu Xie
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摘要

细菌性感染已在临床上使用多种抗生素治疗。然而,目前抗生素的过度使用,由于细菌菌株的出现,造成了严重的耐药性。抗生素使用管理不当导致环境污染,威胁到人们的生命。因此,开发能够将耐药风险降至最低的新型抗菌药物变得至关重要。随着纳米材料和纳米技术在我们日常生活中几乎无处不在,人们对纳米颗粒的抗菌性能进行了研究,并在处理不同菌株的细菌时显示出了良好的效果。含金属的纳米材料虽然非常有效,但可能在人体内积累并产生细胞毒性。近年来,从天然产物中提取的碳纳米点已显示出相当的抗菌效果,但对人体细胞的细胞毒性低,成本低。在这项研究中,研究了枣源碳纳米颗粒(CNPs)对不同革兰氏阴性或革兰氏阳性菌株的杀菌效果。枣源CNPs对革兰氏阳性菌和革兰氏阴性菌均有较强的抗菌作用。令人印象深刻的是,使用制备的CNPs可以完全抑制本研究中使用的所有细菌菌株的生长。此外,所制备的CNP还被发现是一种检测海水污染的良好传感器。为此,研制了一种检测海水污染的酶试剂盒。因此,CNP作为生物传感器在医学和海水污染检测方面具有很大的潜力。
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Effects of Carbon Nanoparticles on Bacteria and Application Potential as Biosensors of Pollution Detection of Ocean Water Carbon Nanoparticles as Antibiotics and Biosensors of Ocean Water
Bacterial infections have been clinically treated with variety of antibiotics. The excessive use of antibiotic nowadays, however, has caused severe drug resistance by the emergence of bacteria strains. The improper management of antibiotic usage has led to the contamination of the environment that threatens people's life. Therefore, it becomes critical to develop novel antibacterial agents that will reduce the risk of drug resistance to its minimum. As the nanomaterial and nanotechnology find their ways to anchor on nearly every aspect of our daily life, the antibacterial properties of nanoparticles have been studied and have shown promising effect while treating different strains of bacteria. Metal containing nanomaterials, though very effective, may potentially accumulate in human body and become cytotoxic. Recently, carbon nano dots derived from natural product have shown comparable antibacterial effect but are low cytotoxicity to human cells and cost. In this study, bactericidal effect of dates-derived carbon nanoparticles (CNPs) on survival of different gram-negative or gram-positive strains was tested. Dates-derived CNPs exhibited strong antibacterial effect against both gram-positive and gram-negative bacteria. Impressively, complete inhibition in the growth of all bacterial strains used in this research was achieved using as prepared CNPs. Moreover, the as prepared CNP was discovered as a great sensor to detect the pollution in ocean water. In deed, an enzyme kit was developed for the ocean water pollution detection. Thus CNP has great potential as biosensor both in medicine and pollution detection of ocean water.
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