栝楼子叶外植体离体微繁研究

M. Venkateshwaralu
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引用次数: 0

摘要

从幼年期外植体培养10-15天开始,研究了一种利用三角鱼子叶外植体进行体外微繁的方法[1,2]。制定子叶外植体培养、愈伤组织诱导和通过子叶愈伤组织再生的方案[3,4]。BAP 1.0 ~ 3.0 ~ 5.0 mg/l和NAA 3.0 ~ 4.0 mg/l Kn对多芽形成有促进作用[5,6]。在Ms. Medium培养基上培养16 ~ 20 d分别诱导出绿色致密小芽芽。
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In Vitro Micropropagation Studies Cotyledonary Explants Of Trichosanthes Anguina (L)
A method for Invitro Micropropagation Through Cotyledonary Explants of Trichosanthes anguina was exercised from 10-15 days of young stage explants [1, 2]. Developed a protocol for cotyledonary explants cultures, callus induction, and regeneration through cotyledon callus tissue [3, 4]. Multiple shoot formation was promoted by BAP 1.0 mg/l to 3.0 to 5.0 mg/l and higher combination with NAA 3.0 to 4.0 mg/l Kn [5, 6]. Invitro produced small shoots were green callus induced to cotyledonary callus cultures green compact small shoots buds were induced in 16-20 days cultures respectively on Ms. Medium.
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