冠状动脉微循环原位研究的方法学方法。

Blood vessels Pub Date : 1991-01-01 DOI:10.1159/000158868
W M Chilian, D V DeFily
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引用次数: 10

摘要

由于心肌的厚度和心脏收缩,原位测量冠状动脉微血管参数是困难的。这两个问题都阻碍了冠状动脉微循环的可视化。我们改进了方法方法,使冠状动脉微循环原位研究成为可能。在第一种方法中,微血管可以在跳动的心脏中可视化,使用一种通过制造心脏静止的错觉来补偿心脏运动的制剂。这是通过在每个心脏周期的同一点闪烁一次频闪光源,并使呼吸机与心脏周期同步来实现的。微血管的图像可以使用标准的活体视频显微技术获得。为了可视化心内膜内和心内膜下的微循环,在离体心脏中完成了研究。在这种准备中,可以在心外膜下和心内膜下微循环中测量微血管直径和压力。后一种方法可以深入了解冠状动脉微血管调节的跨壁差异。
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Methodological approaches used for the study of the coronary microcirculation in situ.

Measurements of coronary microvascular parameters in situ are difficult because of the thickness of the heart muscle and cardiac contraction. Both of these problems hamper the visualization of the coronary microcirculation. We have refined methodological approaches that enable the study of the coronary microcirculation in situ. In the first approach, microvessels can be visualized in the beating heart using a preparation that compensates for cardiac motion by creating an illusion that the heart is motionless. This is accomplished by flashing a stroboscopic light source once per heart cycle at the same point in each cycle and synchronizing a ventilator with the cardiac cycle. Images of microvessels can be obtained using standard intravital video-microscopic techniques. To visualize the intramural and subendocardial microcirculation, studies are completed in isolated hearts. In this preparation, measurements of microvascular diameters and pressures can be performed in both the subepicardial and subendocardial microcirculations. This latter approach allows insight into transmural differences of coronary microvascular regulation.

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