First report of citrus virus A in Australia

Citrus virus A (CiVA) was detected for the first time in Australia in a living pathogen collection. Buds were originally collected from a Washington navel field tree prior to 1970 and graft-inoculated onto a Symons sweet orange indicator plant. The virus was detected using conventional and quantitative reverse transcription polymerase chain reactions and high-throughput sequencing. This Australian variant, known as CL223, shares 96.3% (RNA1) and 96.7% (RNA2) nucleotide identity with isolates of CiVA from South Africa and China, respectively. Foliar symptoms of leaf flecking and oak leaf patterns, consistent with detections of CiVA in other regions, were observed on the foliage of the original accession and inoculated indicator plants. Subsequent surveys of an Australian citrus variety collection detected a different CiVA sequence variant in two accessions of Pera sweet orange; this variant had 97% similarity to CL223, the other Australian variant.