A minimally invasive in vivo electroporation method utilizing flexile electrode and microneedle roller

This paper reports a minimally invasive in vivo electroporation method utilizing parylene-based flexible electrode, after a treatment of microneedle device, Dermalroller®.[1] Upon penetrating high-resistance stratum corneum, the sufficient hypodermic electric field can be established under low voltage condition. Benefiting from more uniform electric field under skin, good biocompatibility, and lower applied voltage, the transfection rate is higher and the electroporated skin is less likely to be harmed compared to the commercial device. Furthermore, the low cost and convenient operation offer vast potential for clinical applications.