J.M. Irvine, J.V. Oakes, C.K. Shewmaker, A. Crossway
{"title":"用于检测植物中特定DNA序列的快速筛选","authors":"J.M. Irvine, J.V. Oakes, C.K. Shewmaker, A. Crossway","doi":"10.1016/0735-0651(90)90008-4","DOIUrl":null,"url":null,"abstract":"<div><p>We have developed a simple and quick method (“wick blot”) for detecting the presence of specific DNA sequences i in plants, using radiolabeled DNA probes. The method requires only small amounts of tissue, about 15–25 mg. More than a hundred samples per day can be easily extracted and blotted. It works well on various species and tissues, including leaves, embryos, and callus. The method is ideally suited for screening large numbers of putative transformants, especially populations that have not been screened by prior selection.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"7 2","pages":"Pages 25-31"},"PeriodicalIF":0.0000,"publicationDate":"1990-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(90)90008-4","citationCount":"2","resultStr":"{\"title\":\"A rapid screen for the detection of specific DNA sequences in plants\",\"authors\":\"J.M. Irvine, J.V. Oakes, C.K. Shewmaker, A. Crossway\",\"doi\":\"10.1016/0735-0651(90)90008-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We have developed a simple and quick method (“wick blot”) for detecting the presence of specific DNA sequences i in plants, using radiolabeled DNA probes. The method requires only small amounts of tissue, about 15–25 mg. More than a hundred samples per day can be easily extracted and blotted. It works well on various species and tissues, including leaves, embryos, and callus. The method is ideally suited for screening large numbers of putative transformants, especially populations that have not been screened by prior selection.</p></div>\",\"PeriodicalId\":77714,\"journal\":{\"name\":\"Gene analysis techniques\",\"volume\":\"7 2\",\"pages\":\"Pages 25-31\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0735-0651(90)90008-4\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene analysis techniques\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0735065190900084\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0735065190900084","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A rapid screen for the detection of specific DNA sequences in plants
We have developed a simple and quick method (“wick blot”) for detecting the presence of specific DNA sequences i in plants, using radiolabeled DNA probes. The method requires only small amounts of tissue, about 15–25 mg. More than a hundred samples per day can be easily extracted and blotted. It works well on various species and tissues, including leaves, embryos, and callus. The method is ideally suited for screening large numbers of putative transformants, especially populations that have not been screened by prior selection.
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